il-36β lentivirus (Genechem)
90
Structured Review
Genechem
il-36β lentivirus
Il 36β Lentivirus, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-36%CE%B2+lentivirus/il+36%CE%B2+lentivirus/pmc08743712-177-25-27
Average 90 stars, based on 1 article reviews
Il 36β Lentivirus, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-36%CE%B2+lentivirus/il+36%CE%B2+lentivirus/pmc08743712-177-25-27
Average 90 stars, based on 1 article reviews
il-36β lentivirus - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p"
Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p
Journal: Annals of Translational Medicine
doi: 10.21037/atm-21-5991
Figure Legend Snippet: IL-36β in cancer tissues is downregulated. (A) IL-36β mRNA levels in cancer tissue (T) samples were 0.392±0.070, while the levels in paracancerous tissue specimens (NT) were 1.493±0.113, and the difference was statistically significant (P<0.05). (B) Immunohistochemistry was performed for IL-36β protein detection in tissue samples (magnification, 200×). Positive signals in pancreatic cancer tissue samples (a). No expression in pancreatic cancer tissue specimens (b). Positive signals in paracancerous tissue specimens (c). No expression in paracancerous tissue samples (d). *, P<0.05. IL, interleukin.
Techniques Used: Immunohistochemistry, Expressing
Figure Legend Snippet: Positive expression rates for the IL-36β protein in cancerous and paracancerous tissue samples
Techniques Used: Expressing
Figure Legend Snippet: IL-36β overexpression in tumor cells inhibits tumor growth in vivo. In total, 1×106 Panc02-Control, Panc02-NC, and Panc02-IL-36β cells were injected subcutaneously into C57BL/6 mice, and the tumor sizes were monitored every other day. Data (mean ± SEM) are from three independent experiments (n=5/group). Until the end of the study, the tumor diameter was markedly reduced in the Panc02-IL-36β group (9.45±0.36 mm) compared with the Panc02-Control (16.59±0.08 mm) and Panc02-NC (17.01±0.65 mm) groups (P<0.05). IL, interleukin; NC, negative control; SEM, standard error of mean.
Techniques Used: Over Expression, In Vivo, Control, Injection, Negative Control
Figure Legend Snippet: Tumoral IL-36β overexpression enhances type 1 immune responses in the tumor microenvironment. On day 33, tumor samples were obtained to generate single-cell suspensions. (A) Percentages of CD45+ cells in tumor cell suspensions. (B,C) Representative flow-cytograms and CD8+ T or NK cell rates within tumor CD45+ cells. *, P<0.05. IL, interleukin; NK, natural killer.
Techniques Used: Over Expression
Figure Legend Snippet: Adenovirus with IL-36β overexpression inhibits tumor growth after intratumoral injection. In total, 1×106 Panc02 cells were administered by subcutaneous injection into C57BL/6 mice. On the 7th day, the animals were randomized into three groups and injected separately with 1×109 PFU IL-36β overexpression adenovirus, 1×109 PFU adenovirus empty vector, and 100 μL PBS. Tumor sizes were monitored every other day. Data (mean ± SEM) are from three independent experiments (n=5/group). Until the end of the study, the tumor diameter was markedly reduced in mice injected with IL-36β adenovirus (11.82±1.28 mm) compared with the control PBS (16.8±1.57 mm) and adenovirus empty vector (16.31±1.40 mm) groups (P<0.05). IL, interleukin; PFU, plaque-forming units; PBS, phosphate-buffered saline; SEM, standard error of mean.
Techniques Used: Over Expression, Injection, Plasmid Preparation, Control, Saline
Figure Legend Snippet: Adenovirus with IL-36β overexpression induces CD8+T, NK, and γδT cell aggregation. On day 27, tumor resection was performed to generate single-cell suspensions. (A) Percentages of CD45+ cells in tumor cell suspensions. (B,C) Representative flow-cytograms, and CD8+ T, NK1.1+, or γδT cell rates among tumor CD45+ cells. *, P<0.05. IL, interleukin; NK, natural killer.
Techniques Used: Over Expression
Figure Legend Snippet: Heat map of 21 miRNAs generated with the R software (pheatmap package). (A) Human; (B) mouse. Myeloid Differentiation Factor 88 (MYD88, a critical upstream effector of IKK/NF-κB signaling). (C) The results of qRT-PCR indicated that let-7c-5p downregulation was the most consistent. Control group, WT CD8+; IL-36β group, WT CD8+/IL-36β stimulation (***, P<0.0001). KO, knockout; qRT-PCR, quantitative real-time PCR; IKK, IKappaBbeta kinase; NF-κB, nuclear factor-kappaB; IL, interleukin; WT, wild type.
Techniques Used: Generated, Software, Quantitative RT-PCR, Control, Knock-Out, Real-time Polymerase Chain Reaction
Figure Legend Snippet: IL-36β downregulates IFN-γ in let-7c-5p+ CD8+ T cells. Data are mean ± SEM. ***, P<0.001 (two-tailed unpaired Student’s t-test). IL, interleukin; IFN, interferon; SEM, standard error of mean.
Techniques Used: Two Tailed Test
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T cells by downregulating micro-RNA let-7c-5p Article Snippet: Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Over Expression:Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Cell culture Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% In Vivo:Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Cell culture Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Control:Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Cell culture Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Injection:Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Cell culture Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Negative Control:Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Cell culture Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Plasmid Preparation:Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Cell culture Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Saline:Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Cell culture Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Article Title: IL-36β promotes anti-tumor effects in CD8 + T 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Quantitative RT-PCR:Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Cell culture Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Knock-Out:Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Cell culture Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Real-time Polymerase Chain Reaction:Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Cell culture Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Two Tailed Test:Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Cell culture Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% Article Title: IL-36β promotes anti-tumor effects in CD8 + T cells by downregulating micro-RNA let-7c-5p Article Snippet: Panc02 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) plus 10% |